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Image Search Results
Journal: PLoS ONE
Article Title: Responses of a Triple Mutant Defective in Three Iron Deficiency-Induced BASIC HELIX-LOOP-HELIX Genes of the Subgroup Ib(2) to Iron Deficiency and Salicylic Acid
doi: 10.1371/journal.pone.0099234
Figure Lengend Snippet: A, At3g07720; B, CYP82C4 ; C, At3g12900; D, MTPA2 ; E, PPC1 ; F, LHY1 ; G, At1g07050; H, PSAF ; The genes in A–E were identified as potential downstream targets of bHLH subgroup Ib(2) factors, while the genes in F–H indicated a more intense response to –Fe in the mutant (compare to ). 3xbhlh and wild type seedlings were grown for 6 d at + and −Fe and exposed for 6 h to 100 µM SA (+SA) or were mock-treated (−SA). Whole seedlings were harvested for analysis. n = 3; the –Fe cDNA samples were derived from the RNAs used in the microarray ; * indicates a significant change (p<0.05) of −Fe versus +Fe; + indicates a significant change (p<0.05) of 3xbhlh versus WT; § indicates a significant change (p<0.05) of +SA versus –SA. Gene expression was studied using reverse transcription-qPCR.
Article Snippet:
Techniques: Mutagenesis, Derivative Assay, Microarray, Gene Expression, Reverse Transcription